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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+synthesis+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11018 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD
    Article Snippet: .. Reverse transcription was carried out using a commercial cDNA synthesis kit (Takara, Shiga, Japan). ..

    Article Title: Integrative transcriptomic analysis revealed the roles and prognostic value of ion channels in hypertrophic cardiomyopathy
    Article Snippet: Total RNA was extracted from mouse heart tissues using TRIzol reagent (Invitrogen, 15596026). .. According to the manufacturer’s protocol, RNA was reverse-transcribed into cDNA using a cDNA Synthesis Kit (TaKaRa, RR036A). .. The resulting cDNA was used as the template for real-time quantitative PCR with TB Green Premix (TaKaRa, RR420A) following the manufacturer’s instructions.

    Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome?
    Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples (Mir-X miRNA First-Strand Synthesis Kit, Takara, Takara Bio Inc., Kusatsu, Shiga, Japan). .. The reverse transcription was performed in a thermocycler (My Cycler, (Bio-Rad Laboratories, Hercules, CA, USA) with an incubation step for 1 h at 37 ◦C; then the reaction was stopped by heating the mixture for 5 min at 85 ◦C, to inactivate the enzymes, prior to a final hold at 4 ◦C. https://doi.org/10.3390/biomedicines14051094

    cDNA Synthesis:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Manganese Could Indirectly Promote Generation and Propagation of the Yeast Prion [URE3] and Increase Molecular Chaperones Expression in Budding Yeast
    Article Snippet: .. First-strand complementary DNA synthesis was performed with hexamers as primers using a cDNA synthesis kit (TaKaRa) (Kusatsu, Shiga, Japan) following the manufacturer’s protocol. .. Quantitative real-time PCR was performed using the SYBR Premix Ex Taq II kit (TaKaRa) and the Roche LightCycle 480 II sequence detection system (Roche, Switzerland) (Basel, CHE).

    Article Title: Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD
    Article Snippet: .. Reverse transcription was carried out using a commercial cDNA synthesis kit (Takara, Shiga, Japan). ..

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.

    Article Title: Dietary supplementation with Bacillus velezensis enhances the intestinal health of broiler chickens challenged with necrotic enteritis via reshaping the structure and function of the intestinal flora
    Article Snippet: .. Then, the gDNA Eraser (Takara, Dalian, China) was used to remove contaminating genomic DNA, and cDNA synthesis kit (Takara, Dalian, China) was used to reverse it into stabilized cDNA. .. The stable cDNA, the primers for the target gene, and the fluorescent dye were mixed and tested on the fluorescence quantitative PCR instrument (an applied biosystems 7500 fast Real-time PCR system, Foster City, CA).

    Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome?
    Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples (Mir-X miRNA First-Strand Synthesis Kit, Takara, Takara Bio Inc., Kusatsu, Shiga, Japan). .. The reverse transcription was performed in a thermocycler (My Cycler, (Bio-Rad Laboratories, Hercules, CA, USA) with an incubation step for 1 h at 37 ◦C; then the reaction was stopped by heating the mixture for 5 min at 85 ◦C, to inactivate the enzymes, prior to a final hold at 4 ◦C. https://doi.org/10.3390/biomedicines14051094

    Quantitative RT-PCR:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.

    Real-time Polymerase Chain Reaction:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.

    DNA Synthesis:

    Article Title: Manganese Could Indirectly Promote Generation and Propagation of the Yeast Prion [URE3] and Increase Molecular Chaperones Expression in Budding Yeast
    Article Snippet: .. First-strand complementary DNA synthesis was performed with hexamers as primers using a cDNA synthesis kit (TaKaRa) (Kusatsu, Shiga, Japan) following the manufacturer’s protocol. .. Quantitative real-time PCR was performed using the SYBR Premix Ex Taq II kit (TaKaRa) and the Roche LightCycle 480 II sequence detection system (Roche, Switzerland) (Basel, CHE).

    Synthesized:

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.

    Amplification:

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    Polymerase Chain Reaction:

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    Gene Expression:

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.

    Isolation:

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.

    SYBR Green Assay:

    Article Title: Autophagy-lysosomal dependency defines a vulnerable physiological state in drug-tolerant persister cells of triple-negative breast cancer.
    Article Snippet: 1 Department of Biological Sciences, National Institute of Pharmaceutical Education and Research, Balanagar, Hyderabad 500037, India 2 Dept. of Biotechnology and Bioinformatics, Sambalpur University, Jyoti Vihar, Burla, Odisha 768019, India 3 Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India 4 Council of Scientific and Industrial Research (CSIR)-Centre for Cellular and Molecular Biology, Hyderabad 500007, India Abstract Triple-negative breast cancer (TNBC), a subtype of aggressive breast cancer, has limited treatment options.. Recurrent disease caused by drug-tolerant persister cells (DTPs) that evade chemotherapeutic agents (e.g., doxorubicin hydrochloride (DOX) and paclitaxel (PTX)) is a significant challenge in treating TNBC.. Recent studies highlight both autophagy and lysosomal function as key mechanisms supporting cancer cell survival; however, their precise roles in mediating drug tolerance in TNBC remain largely unexplored.



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